Since it was first described and demonstrated in 2014, lattice light-sheet microscopy has been exciting the research community. This technology combines the gentleness of conventional light-sheet imaging with the high resolution of confocal microscopes, allowing living samples to be imaged over longer periods of time and with subcellular resolution.
To create a lattice light-sheet image, the sample is illuminated perpendicular to the direction of observation so that only those areas of the sample in the focal plane are exposed to the damaging effects of light. Innovations have allowed for the generation of thinner light sheets, significantly improving resolution in z, while retaining the advantages of traditional light-sheet microscopy—fast volumetric imaging with minimal phototoxicity.
The combination of both high spatial and temporal resolution, rapid imaging, and unrivaled gentleness makes lattice light-sheet microscopy the preferred choice for live-cell imaging. In this webinar, our experts will introduce the viewer to this enabling technology, highlighting its development, utility, and application.
University of California, Berkeley/HHMI
KTH Royal Institute of Technology
Carl Zeiss Microscopy GmbH
- Learn about the principles of lattice light-sheet microscopy and its advantages for 3D imaging of subcellular dynamics over time
- Gain insights into the development process for this technique
- See how first-time users have successfully implemented this technology to address critical questions in life sciences