From 3D Light to 3D Electron Microscopy - Science/AAAS Ebook
FREE Science/AAAS Ebook Reveals

From 3D Light to 3D Electron Microscopy

Shared experiences from the volume EM community

In this new 28-page Science/AAAS ebook, learn more about volume electron microscopy (vEM) and volume correlative light and electron microscopy (vCLEM) for life sciences.

Get an introduction to the community, read articles and watch videos about vEM and vCLEM workflows, and get inspired from a discussion among electron microscopy experts from both academia and industry.

  • Introduction to vEM/vCLEM and its community
  • Selected articles, describing vEM and vCLEM workflows
  • Panel discussion among electron microscopy experts from both academia and industry

Here's How The eBook Looks Inside

When You Download

  • From 3D Light to 3D Electron Microscopy - Ebook Preview 1
  • From 3D Light to 3D Electron Microscopy - Ebook Preview 2
  • From 3D Light to 3D Electron Microscopy - Ebook Preview 3
  • From 3D Light to 3D Electron Microscopy - Ebook Preview 4

Table of Contents

Sharing the science behind 3D electron microscopy

Sean Sanders
(Science/AAAS, Washington, DC, USA)

Community under the microscope

Alexandra Elli, Daniel Hartmann, and Bernhard Zimmermann
(Carl Zeiss Microscopy GmbH, Jena, Germany)

Sample preparation for volume correlative light and electron microscopy

Jean Daraspe, Olivia Muriel, Sophie G. Martin, Christel Genoud
(UNIL, EMF, Lausanne, Switzerland)

Breaking down the correlative workflow for array tomography:
From section collection to image analysis

Ian J. White1, Steve Furzeland2 Jemima J. Burden1
(1UCL, LMCB, London, UK, 2ZEISS RMS, Cambourne, UK))

X-ray targeting of Arabidopsis pollen tetrads for SBF-SEM

Anna Kremer1, Kimberly Meechan2, Marianne Beckwith2, Saskia Lippens1
(1VIB, Ghent, Belgium, 2EMBL, Heidelberg, Germany)

Cryo-correlative light and electron microscopy for cellular tomography

Anna M. Steyer, Zhengyi Yang, Simone Mattei
(EMBL, Heidelberg, Germany)

Fluorescence-based targeting of a plankton cell for FIB-SEM

Karel Mocaer, Giulia Mizzon, Manuel Gunkel, Aliaksandr Halavatyi, Yannick Schwab, Paolo Ronchi
(EMBL, Heidelberg, Germany)

Introducing high-throughput serial section acquisition for ZEISS MultiSEM

Anna Lena Eberle, Tomasz Garbowski, Stephan Nickell
(Carl Zeiss MultiSEM GmbH, Oberkochen, Germany)

Panel discussion: vEM/vCLEM community building

Johanna Bischof (Euro-BioImaging Bio-Hub, EMBL, Germany), Lucy Collinson (The Francis Crick Institute, UK), Kedar Narayan (NIH-NCI, USA), Yannick Schwab (EMBL, Germany), Paul Verkade (University of Bristol, UK), Alexandra Elli (Carl Zeiss Microscopy, Germany), Rosy Manser (Carl Zeiss Microscopy, UK)

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