Web Demo For APAC Customers

LSM Lightfield 4D

Instant Volumetric High-Speed Imaging of Living Organisms

Lightfield 4D is instant volumetric imaging at high speed. Acquire comprehensive 3D information with a single snap and say goodbye to any time delay within an imaged volume. For the first time, capture the fastest movements within whole organisms at up to 80 volumes per second – with all spatiotemporal information intact. Crawling larvae, beating hearts, flowing blood, and firing neurons can be studied in 3D at unprecedented speed to unravel the secrets of life.

Join us for from May 6 - 16 for our exclusive ZEISS Lightfield 4D Web Demo to experience the speed of volume imaging.

One Snap. One Volume.

Capture Spatial Signals and Fast Dynamics without Compromise

Investigating the morphology and cardiac wall movement of the developing zebrafish heart. Courtesy of Stone Elworthy and Emily Noël, School of Biosciences, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.

Life moves. Many neuronal and physiological processes occur at very high speeds, making it difficult to accurately capture their spatiotemporal dynamics. Although established technologies have become faster, the required acquisition time still increases with sample volume, so fast processes like neuronal activity or heartbeats require a trade-off between volumetric information and image frame rate. With Lightfield 4D, you no longer have to compromise, as you can capture 80 volumes per second without time delay in 3D.

This makes it possible to follow neuronal activity in zebrafish brains, track tissue movement in developing Drosophila embryos, and keep track of moving structures in crawling C. elegans larvae. The unique one-snap-one-volume imaging ensures that crucial events are not missed or distorted. Highly time-resolved particle tracking in complete volumes is finally possible. Start your experiments immediately – on your confocal and without the need to adjust sample preparation.

Minimum Light Exposure. Maximum Information Gain.

Observe Entire Organisms for as Long as You Want Without Altering the Processes of Life.

Observing the formation of fat body tissue in a developing Drosophila pupa. Courtesy of Ignacio Manuel Fernández Guerrero, Cellular Analysis Facility, MVLS-Shared Research Facilities, University of Glasgow. Data acquired at the Cellular Analysis Facility, University of Glasgow

Collecting 3D information of living samples has always been a challenge, especially for large sample volumes. Optical sectioning requires sequential acquisition of single images to create a Z-stack. Each slice requires light exposure, which is not fully limited to the plane of illumination and can easily add up to harmful amounts across the volume. Lightfield 4D works differently: A complete Z-stack is acquired with a single illumination event, reducing light exposure and phototoxic effects to a minimum. Living samples can be imaged over long periods of time at high temporal density.

This combination of outstanding 3D imaging speed and extreme gentleness allows you to follow the sample in multicolor over time without influencing the recorded living activity. You can observe developmental processes, cell migration, vesicle movement or other changes in tissues and organisms that take hours or even days to complete, and still achieve the temporal resolution needed to understand the dynamics.

Fast Acquisition. Increased Throughput.

Examine Multiple Positions or Numerous Samples with Instantaneous Volumetric Imaging.

Efficient volume imaging of cleared spheroids with subsequent cell counting. Sample courtesy of InSphero AG. Schlieren, Switzerland

Typically, acquisition time of large volumes is the critical factor that limits the throughput of imaging. Acquiring a large volume with a single image snap speeds up your experiments by multitudes. The unmatched speed with which Lightfield 4D captures multi-color volumes can be used to increase the productivity of experiments in a variety of ways: Image and analyze more samples than ever before in every session, immediately improving experimental statistics. Compare multiple different sample cohorts of wild type and genetically modified phenotypes, or samples with different drug treatments. Instead of hours, only minutes are spent collecting the data you need, leaving you more time for advanced analysis and investigation of your datasets.

Register for the FREE web demo below

Learn how ZEISS Lightfield 4D can be implemented in your workspace

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Registration is only accepted with a university, institute, or company email address.

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The first series of web demos will take place from May 6 to 16. Please select your two preferred dates.

LSM Lightfield 4D

One Snap. One Volume.

Minimum Light Exposure. Maximum Information Gain.

Fast Acquisition. Increased Throughput.

Register for the FREE web demo below

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