NUSNNI - ZEISS Seminar and ORION NanoFab Workshop

NUSNNI - ZEISS Seminar and ORION NanoFab Workshop

27 - 28 February 2019

ZEISS on your Campus (ZOYC) | 27 - 28 February 2019 08:45 AM - 5:15 PM | National University of Singapore - T-lab Building

NUS Nanoscience & Nanotechnology Initiative (NUSNNI) - ZEISS Seminar and Workshop

Nanoscale Structuring and Imaging of Materials

Nanofabrication is the design and manufacturing of devices with dimensions measured in nanometers. To use the advantages of nanotechnology, you need to create small structures. Charged particles like ions or electrons are often your method of choice. The interaction between the ion or electron beam and the sample surface allows you to manipulate structures or properties of the surface. When used in combination with different gases, you are able to perform complex processes such as etching or material deposition. This enables creation of superior new materials and systems with complex mechanical, electronic, optical, magnetic or fluidic functions.

This educational seminar is a partnership between NUSNNI and ZEISS. It will bring insights into the latest technology regarding Microscopy (Light, Electron, X-ray and Ion) and Nanofabrication for both Materials Scientists and Biologists. 

Day 1 | Educational Seminar

  • Schedule
    8:45 AM
    Welcome note
    9:00 AM
    Materials Issues in Battery Technology
    Dr. Stefan Adams, National University of Singapore
    9:30 AM
    Imaging & Analysis of Batteries
    Dr. Jaehan Lee, ZEISS
    10:00 AM
    Focused Ion Beam systems - A brief history of the technology
    Beam Microscopy: Evolution and Application

    Dr. Venkatesan Thirumalai Venky, National University of Singapore
    10:30 AM
    Coffee break
    11:00 AM
    Advances in Microscopy for Material Science Research
    Dr. Hanfang Hao, ZEISS
    11:30 AM
    Cutting Edge of STEM
    Dr. Stephen John Pennycook, National University of Singapore
    12:00 NN
    Advanced Characterization & Nanofabrication Using He & Ne Ions, with the ZEISS ORION NanoFab
    Dr. Vignesh Viswanathan, ZEISS
    12:30 PM
    1:30 PM
    NUSNNI Poster Session
    2:30 PM
    Bio-inspired Flexible and Stretchable Electronic Skins
    Dr. Benjamin C.K. Tee, National University of Singapore
    3:00 PM
    Next Generation Sensor Devices
    Dr. Chengkuo Lee, National University of Singapore
    3:30 PM Coffee break
    4:00 PM
    Electron Microscopy: A Fantastic Tool for Biologists
    Dr. Isabelle Bonne, National University of Singapore
    4:30 PM 3D SEM Technique for Bio-Medical Research
    Dr. Philipp Bastians, ZEISS
    5:00 PM Closing Note
    Tonmoy Kundu, ZEISS
    5:15 PM End of Seminar 
  • Speakers & abstracts

    9:00 AM // Dr. Stefan Adams, Associate Professor, Department of Materials Science & Engineering
    National University of Singapore
    Materials Issues in Battery Technology

    Major breakthroughs in battery technologies are needed for electric vehicles that could safely drive longer on a single charge and recharge promptly. At the same time the deployment of wind and solar power for a sustainable economy requires powerful low-cost large-scale batteries with long cycle-life made from sustainable materials resources. It is obvious that the maturing Li-ion battery (LIB) technology relying on costly cathode materials, flammable electrolytes with limited electrochemical stability windows and bulky anodes with limited rate capabilities cannot offer the performance levels required for these key applications. In this talk, current R&D trends in overcoming these challenges will be outlined.

    Back to the future - From Li-ion batteries to Li metal batteries: One of the most promising approaches to enhance the energy density and rate capability is to move back from LIBs to batteries that use metallic Li instead as the negative electrode. Replacing conventional graphite or oxide anodes with Li metal opens up a pathway to gravimetric energy densities up to 400 Wh/kg and volumetric energy densities approaching 1000 Wh/L. This approach had been abandoned a few decades ago due to the lack of electrolytes that at that time were known to be stable in contact with metal anodes and high voltage cathodes as well as the hazard of short-circuits by Li dendrites. Recent progress on solid electrolytes raises expectations that these could enable Li-metal batteries by acting as artificial anode protecting solid-electrolyte interfaces and mechanical barriers against dendrite growth.

    Quidquid agis ... respice finem – focus on sustainable and scalable designs: For stationary large-scale applications where low cost and volumetric energy density gain importance, analogous strategies are pursued for Na-based batteries, which of course only makes sense if the low-cost high-performance anodes are combined with affordable, scalable cathodes, where high-cost transition metal compounds (e.g. Cobalt) are replaced by earth-abundant cathode materials.

    Nihil nisi solidum - From liquid electrolytes to all-solid state batteries: While compatibility with current LIB fabrication may initially favour the replacement of anodes by Li-metal : anode-protecting membrane combinations, it appears natural to get rid of flammable liquid electrolytes altogether in order to enhance safety, (volumetric) energy density as well as reduce the need for enclosure of individual cells and enable longer cycle life systems. While data of individual solid electrolytes imply that this should be within reach, the key challenge remains to be the realisation of electrochemically stable electrolyte:electrode interfaces that ensure smooth charge transfer over a long cycle life despite the volume changes on cycling.

    Getting the full picture - From materials design to system design: To overcome performance bottlenecks at system level it is widely recognised that the research focus has to shift from individual materials with record performance to a rational design of devices, which mainly involves the identification and engineering of favourable electrode:electrolyte combinations. Major progress can be expected in this area from combining the emerging experimental in situ and operando characterisation technologies with computational approaches.

    Do What You Do Best: Moving from computer-aided to AI design: Analysing the wealth of complex experimental data e.g. from operando experiments or computational characterisations of a vast number of potential materials combinations and matching them to requirement profiles tailored for specific applications with an open mind for novel battery architec¬tures should be a fitting artificial intelligence problem. Thus, the most effective way to accelerate the develop¬ment of high performance energy storage systems may be to set up a dedicated battery design system and feed it with dependable standardised data, leaving the creative design to the AI tool. 


    About the speaker

    Stefan Adams, PhD

    After his Ph.D. from Saarbrücken/Germany, Stefan Adams worked at the Max-Planck Institute for Solid State Research in Stuttgart and completed his habilitation at Göttingen University before joining National University of Singapore in 2005, where he is now Assoc. Professor for Materials Science and Engineering as well as Adjunct Researcher at the A*Star Institute for Materials Research and Engineering (IMRE) and the NUS Centre for Advanced 2D Materials. Adams serves the Asian Society for Solid State Ionics as their Secretary, the Materials Research Society of Singapore as Joint Secretary and is a member of the Singapore National Committee for Crystallography. He is Editorial Board member of several journals incl. Acta Crystallographica B, Solid State Ionics, Ionics etc. His research interests focus on the design of structures and interfaces with optimized transport properties in nanostructured oxides and chalcogenides for advanced energy storage and conversion devices.

    read abstract

    9:30 AM // Dr. Jaehan Lee, Product Marketing Manager, Business Sector Electronics, ZEISS

    Imaging & Analysis of Batteries

    Since the commercialization of lithium-ion battery(LIB) by Sony in 1991, LIB has been the most widely used energy storage device for smart phones, laptop computers, internet of things(IOT), and electric vehicles(EVs). To meet the megatrend of widespread LIB usage, higher capacity and safety must be achieved for long-term operation and stability. Multimodal/multiscale microscopic characterization based on Zeiss light, electron and X-ray microscopy(XRM) systems, combined with atomic force microscopy(AFM), energy-dispersive X-ray spectroscopy(EDS) and Raman systems from the atomic level to the microscale level, is employed to elucidate structural origins and electrical properties of enhanced battery performance and safety.

    High resolution imaging and microstructure characterization using Gemini SEM and Crossbeam(FIB): The electrochemical performance of LIB, such as its cyclability and power capability, is highly dependent on electrodes, separator and electrolyte. The high-resolution imaging of main active materials helps to explore what is going on inside the battery with regards to by-products spreading, surface electrolyte interface(SEI) formation, dendrite growth and structural changes. This type of information enables a researcher to understand how the product is operating under the intended service condition and develop the better performance LIB.

    Electrical property measurement techniques using Hybrid SEM & AFM, Micro-probing system and Electron Beam Absorption Current(EBAC) systems: The performance of LIB is intrinsically linked to the microstructural and electrical properties of its materials. The unit cell consists of many electronics materials such as current collectors, conducting agent, layered graphite and lithium metal oxide particles to utilize electrons during chemical reactions. Electrical properties such as conductivity, electrical potential, and work function on battery materials and cells are the key information to develop higher quality materials and better performance cells along with customized formulations.

    Non-destructive, three-dimensional and multiscale measurements using X-ray microscopy(XRM) system: Advances in XRM Versa systems have enabled non-destructive and three-dimensional microstructural characterization to sub-micron resolution in a unique optics and detector design. This invention allows greater flexibility than conventional computed tomography(CT) system, enabling multi-length scale measurement and volumetric change study that range from the observation of cell swelling and deformation to degradation phenomena of electrode microstructures of LIBs.

    About the speaker

    Jaehan Lee

    Jaehan Lee is a solution manager in Business Sector Electronics, Carl Zeiss GmbH. He studied Mechanical engineering as well as Optics and Photonics at Karlsruhe Institute of Technology (KIT), Germany. He worked for ASML and Samsung to develop electrical property measurement techniques for electronics materials and devices from 2004. He worked as a senior researcher in the Samsung R&D center and was mainly responsible for open innovation projects to develop advanced characterization tools and methods for lithium ion batteries and thin film electronics devices. In 2017, he was a visiting scientist at Zentrum fuer Sonnenenergie- und Wasserstoff-Forschung(ZSW) Germany to research advanced LIB materials for EVs.

    read abstract

    10:00 AM // Dr. Venkatesan Thirumalai Venky, Professor of Electrical and Computer Engineering and Physics Faculty of Engineering
    National University of Singapore
    Focused Ion Beam systems - A brief history of the technology and where we are headed in the future

    In this talk I will give a brief overview of the development of focused ion beam (FIB) systems starting with liquid metal ion sources to the current field emission sources. I will start with some of the early developments at Bell Labs where I worked on the development of a liquid metal ion source based focused ion beam systems and finding suitable applications for this technology. I will give examples of a number of material modification where the FIB systems could be of value.

    The use of these ion beam systems can be classified largely in to materials modification, lithography and high resolution imaging. I would like to give some examples from the early work done at NUS on our first Helium ion microscope system and speculate on futuristic experiments.

    About the speaker

    Prof. T. Venkatesan

    Prof. T. Venkatesan is currently the Director of the Nano Institute at the National University of Singapore (NUSNNI) where he is a Professor of ECE, Physics, MSE and NGS. He wore various hats at Bell Labs and Bellcore before becoming a Professor at University of Maryland. As the inventor of the pulsed laser deposition (PLD) process, he has over 760 papers and 30 patents and is globally among the top one hundred physicists (ranked at 66 in 2000) in terms of his citations (Over 43,800 with a hirsch Index of 106 - Google Scholar). He has graduated over 45 PhDs, 35 Post Docs and over 35 undergraduates. He is also the founder and Chairman of Neocera, a company specializing in the area of PLD and magnetic field imaging systems. Close to 10 of the researchers (PhD students and Post Docs) under him have become entrepreneurs starting over 17 different commercial enterprises. He is a Fellow of the APS, winner of the Bellcore Award of excellence, Guest Professor at Tsinghua University (China), Winner of the George E. Pake Prize awarded by American Physical Society (2012), President’s gold medal of the Institute of Physics Singapore, Academician of the Asia Pacific Academy of Materials, Fellow of the World Innovation Forum, was a member of the Physics Policy Committee (Washington DC), the Board of Visitors at UMD and the Chairman, Forum of Industry and Applications of Physics at APS. He was awarded the outstanding alumnus award from two Indian Institute of Technologies- Kanpur (2015) and Kharagpur (2016), India.

    read abstract

    11:00 AM // Dr. Hanfang Hao, NanoFab Business Development, Material E.M. Specialist, ZEISS Research Microscopy Solutions
    Advances in Microscopy for Material Science Research

    Innovations in microscopy instruments and workflows enable the development of novel materials in various research fields. These new class of materials require advanced imaging and characterization tools to understand the microstructure and related functional properties for subsequent optimization in different applications.

    Electron microscopy has become the workhorse technique in the advanced material research with the development made in electron optical components and detector technologies enabling superior resolution and performance even under stringent beam sensitive conditions. Novel technologies incorporating multiple electron beams, up to 91 beams, have revolutionized the scale and speed of analysis over several orders of magnitude. These new developments have enabled high speed inspection and failure analysis of materials and components.

    With the addition of focused ion beam columns, the characterization can be extended to 3D volume. Simultaneous imaging and analytical characterization of materials in 3D in a fully automated manner are providing a wealth of information previously inaccessible with other techniques. Furthermore, ZEISS will also provide the fastest solution in the market for the high-resolution analysis of deeply buried structure with our newly developed femtosecond laser option.

    Connected microscopy is another key concept in advanced microscopy. Combining the electron microscopy tools with non-destructive 3D characterization using x-ray microscopy has transformed existing workflows and methods of studying materials and devices. Diffraction contrast tomography, in-situ studies and correlative workflows combining the 3D non-destructive X-ray microscopy with high resolution FIB-SEM tomography are key developments that are addressing the multi-scale challenges in studying these materials.

    About the speaker

    Hanfang Hao, PhD

    Hanfang Hao is the Materials Electron Microscopy specialist with ZEISS Research Microscopy Solutions, Asia Pacific. Prior to ZEISS, Hanfang worked as a research scientist on Dielectric Nano-antennas design fabrication and characterization at Data storage Institute, A*STAR in Singapore. She received her PhD degree in Electrical and Computer Engineering from the National University of Singapore with the focus on plasmonic nanostructure direct-patterning using focused ion beams. She has been working with various ZEISS microscopes at ZEISS demo lab (previously located in NUS) since 2009. Hanfang also supports ion microscope activities in this region including Crossbeam and NanoFab.

    read abstract

    11:30 AM // Dr. Stephen J. Pennycook, Professor of Materials Science & Engineering
    National University of Singapore
    Cutting Edge of STEM

    The aberration-corrected scanning transmission electron microscope (STEM) provides real space imaging and spectroscopy with unprecedented sensitivity down to the single atom level. Thoroughly understanding and tailoring structural defects is extremely significant for understanding the structure-property relations of existing high-performance materials, and more importantly, guiding the design of new materials with improved properties. Several representative studies will be presented.

    In 2D materials, sub-Ångstrom information transfer can be achieved at only 40 kV, and point defects and their local environments directly identified to correlate with properties. New edge structures in nanoporous MoS2 are found to exhibit excellent catalytic properties [1], and 2D Mo metal membranes can be fabricated from MoSe2 films via beam induced sputtering of Se [2].

    In piezoelectrics, the development of lead-free materials with enhanced properties is urgently required. Precise mapping of atomic displacements reveals a hierarchical nanodomain structure as the origin of excellent properties, the coexistence of ferroelectric phases inside nanodomains and gradual polarization rotation between them [3,4]. Similarly, in thermoelectrics, a hierarchical structure ranging from point defects through nanoscale and microscale precipitates results in a high-performance material with lattice thermal conductivity approaching the theoretical minimum [5].

    In oxide thin films, interplay of octahedral rotations, charge transfer and interdiffusion has major influence on carrier density and mobility and magnetic properties. Atomic resolution mapping of atomic displacements provides the most fundamental understanding of ferroelectric properties.

    Finally, recent progress in optical sectioning to reveal the 3D structure of extended defects will be presented.

    [1] X. Zhao, et al., Nano Lett, 18 (2017) 482–490.
    [2] X. Zhao, et al., Adv. Mater. (2018) 1707281.
    [3] T. Zheng, et al., Energy Environ. Sci, 10 (2017) 528–537.
    [4] B. Wu, et al., J Am Chem Soc, 138 (2016)15459–15464.
    [5] Y. Xiao, et al., J Am Chem Soc, 139 (2017) 18732–18738.


    About the speaker

    Stephen J. Pennycook

    Stephen J. Pennycook is a Professor in the Materials Science and Engineering Dept., National University of Singapore, an Adjunct Professor in the University of Tennessee and Adjoint Professor in Vanderbilt University. He is a Fellow of the American Physical Society, the American Association for the Advancement of Science, the Microscopy Society of America, the Institute of Physics and the Materials Research Society and has received the Microbeam Analysis Society Heinrich Award, the Materials Research Society Medal, the Institute of Physics Thomas J. Young Medal and Award and the Materials Research Society Innovation in Characterization Award.

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    12:00 NN // Dr. Vignesh Viswanathan, Product & Applications Sales Specialist
    ZEISS Research Microscopy Solutions
    ORION NanoFab – Tool for Advanced Characterization & Nanofabrication

    The ORION NanoFab is an imaging, nanofabrication and characterization platform based on a high brightness and stable Gas Field Ion Source (GFIS). The GFIS employed exhibits a low energy spread, small virtual source size and a high brightness to produce fine probes of Helium and Neon ion beams. This, in conjunction with the shallow escape depth (<1 nm) of the secondary electrons generated by the incident ions, contribute to the high spatial resolution achievable with this technology. In addition to the high resolution, the integrated electron flood gun enables imaging of insulating samples without any conductive coating without charging artifacts. This has opened the application of this technology to a broad spectrum of multidisciplinary applications from basic materials science and semiconductor applications to the biological sciences.

    Furthermore, the appreciable sputter yield from helium and neon ions and the small probe size enable material modification and nanopatterning capabilities at the sub-10 nm dimension. Direct milling and material modification with sub-10 nm features on graphene and other 2D materials, plasmonic devices on metals, nanopores and lithography of resists, direct metal and insulator deposition using gas injection systems have already been demonstrated with this tool.

    The sputter process with the primary He or Ne ions results in secondary ions generated from the sample surface like in Secondary Ion Mass Spectroscopy (SIMS). By integrating a custom designed mass spectrometer to this platform, analytical and elemental characterization capabilities have been enabled to add-on to the existing capabilities of the tool. The ability to perform high sensitivity surface analysis and depth profiling with large dynamic range detecting all elements and differentiating isotopes with SIMS complements the surface sensitive imaging observed with this tool. We have demonstrated that our instrument is capable of producing elemental SIMS maps with lateral resolution down to 12 nm. Furthermore, the instruments opens up an in-situ correlative imaging technique combining high resolution SE images and SIMS elemental maps for various applications.


    About the speaker

    Vignesh Viswanathan, PhD

    Dr Vignesh Viswanathan is a Product & Applications Sales Specialist in Materials Electron Microscopy with ZEISS Research Microscopy Solutions, Asia Pacific. Prior to ZEISS, Vignesh worked in Advanced Micro Devices (AMD) performing failure analysis on their leading nodes GPUs and APUs. He graduated from National University of Singapore with a PhD focusing on a novel plasmonics imaging instrumentation and advanced nanofabrication using the Helium Ion Microscope. He specializes in nanopatterning, ion beam lithography and imaging with ORION NanoFab. Vignesh also supports FESEMs, CrossBeam and XRM.

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    1:30 PM // NUSNNI team
    National University of Singapore
    NUSNNI Poster Session

    Contributor Poster Topic
    Cao Yu BTO Thin Films Based Optical Modulators
    Huang Zhen Growth Of STO On Silicon Using Multi-Buffer Layers
    Sonu Devi Free Standing Oxide Heterostructures Using Water Soluble Buffer Layers
    Liang Haidong Smart Cut Process In SrTiO3
    Wan Dongyang Vanadate And Niobate Based Alloy For Transparent Conducting Oxides
    Wan Dongyang Large Polaron Phase Diagram In Anatase Tio2
    Hariom Jani Effect Of Hydrogen Incorporation On Electrical And Magnetic Properties Of Fe2O3
    Saurav Prakash VO2 Based Metasurfaces
    Lim Zhi Shiuh Observation Of Skyrmions In Mn-Ir Based Superlattices
    Zeng Shengwei Manipulation Of Transport In Oxide Heterostructures With Ionic Gating
    Ganesh Ji Omar Large Spin-Orbit Coupling At La Ferrites And STO/LAO Heterostructures
    Viknish Kutty Controlling Cell Growth By Oxide Substrates
    Li Changjian Imaging Dipoles In Ferroelectric Interfaces
    Siddhartha Ghosh Wetting In Rare Earth Oxides
    Zhou Wenxiong Coexistence Of Ferroelectricity And 2D Electron Gas
    Shawn Johannes Siew Oxide Optical Devices
    Teguh Citra Asmara Ion Implantation Effect On The Refractive Index Of SrTiO3
    Meenakshi Annamalai C-AFM Study Of Memory Switching Behaviour In Azo Complex
    Divya Nagaraju In-situ Study Of A Novel Copper Catalyst For CO2 Reduction Reaction
    Soumya Sarkar Magneto-Optical Studies Of Narrow Photoluminescence From Defect States In Rare Earth Oxides
    Sreetosh Goswami Low Energy Switching Organic Memories On Oxides
    read topics & contributors

    2:30 PM // Dr. Benjamin C.K. Tee, President’s Assistant Professor in Materials Science and Engineering Department, National University of Singapore
    Bio-inspired Flexible and Stretchable Electronic Skins

    Electronic sensor skins are active area of research for many groups globally due to its potential to enable dramatic changes in how we interact with the digital environment. For example, ‘robots’ can don on sensor active skins to shake human hands with comfortable pressure, measure our health biometrics and possibly aid in wound healing. In my talk, I will discuss the development of electronic sensor skins with some historical context, followed by showcasing of several force sensitive electronic skin technologies with high sensitivity, stretchability and bio-mimetic self-healing abilities [1-5]. More recently, we demonstrated a power-efficient artificial mechano-receptor system inspired by biological mechano-receptors [5]. We further used a channelrhodopsin with fast kinetics and large photocurrents as an optical interface to neuronal systems for next generation opto-tactile prosthetic interfaces.

    [1] L. Y. Chen*, B. C-K Tee*, et al. Continuous wireless pressure monitoring and mapping with ultra-small passive sensors for health monitoring and critical care. Nature Communications 5, 1–10 (2014). (*equal contribution)
    [2] B. C.-K. Tee, et al. An electrically and mechanically self-healing composite with pressure- and flexion-sensitive properties for electronic skin applications. Nature Nanotechnology 7, 1–8 (2012).
    [3] S. C. B., Mannsfeld, B. C-K Tee et al. Highly sensitive flexible pressure sensors with microstructured rubber dielectric layers. Nature Materials 9, 859–864 (2010).
    [4] Skin-Like Sensors of Pressure and Strain Enabled by Transparent, Elastic Films of Carbon Nanotubes, D.J. Lipomi*, M. Vosgueritchian*, B. C-K. Tee* et al., Nature Nanotechnology, 1–6, (2011)
    [5] A skin-inspired organic digital mechanoreceptor, B. C-K. Tee et al., Science, 350, 313–316, (2015)


    About the speaker

    Benjamin C.K. Tee, PhD

    Dr. Benjamin C.K. Tee is appointed President’s Assistant Professor in Materials Science and Engineering Department at the National University of Singapore. He obtained his PhD at Stanford University, and was a Singapore-Stanford Biodesign Global Innovation Postdoctoral Fellow in 2014. He has developed and patented several award-winning electronic skin sensor technologies. He is an MIT TR35 Innovator (Global) in 2015 and Singapore National Research Foundation (NRF) Fellow. His research group aims to develop technologies at the intersection of materials science, mechanics, electronics and biology, with a focus on sensitive electronic skins that has tremendous potential to advance global healthcare technologies in an increasingly Artificial Intelligence (AI) era.

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    3:30 PM // Dr. Chengkuo Lee, Associate Professor of Electrical and Computer Engineering
    National University of Singapore
    Next Generation Sensor Devices

    The cutting-edge sensing technology is evolving from silicon based microsensors to the nano-antennas based optical sensors and wearable self-powered flexible sensors. Looking into the new market of internet of the things (IoT), we introduce two kinds of promising sensing technologies in this talk. Firstly, “hybrid metamaterial” absorber platform is presented by integrating the nano-antennas based metamaterial with a gas-selective-trapping polymer for highly selective and miniaturized optical sensing of CO2 gas in the 5–8μm mid-IR spectral window. This optical sensor offers a minimum of 40 ppm detection limit at ambient temperature on a small footprint (20 μm by 20 μm), fast response time (≈2 min), and low hysteresis. Secondly, triboelectric nanogenerator (TENG) based self-powered flexible sensors have been investigated as a sensing platform for various applications including 1) amenity sensor for smart home under IoT concept; 2) implantable flexible volume sensor to detect the fullness of the bladder, where we have also demonstrated a self-controlled system for neurogenic underactive bladder by integrating microactuator and flexible volume sensor; 3) a self-powered 3D controller made of liquid-metal and polydimethylsiloxane (PDMS) mixture that deforms and contacts with different sensing electrodes under different applied force. By employing vector properties of force and signal analysis from the eight sensing electrodes, detection of six-axis directions in 3D space is achieved by analyzing triboelectric sensor outputs for the first time. It is capable of generating a triggering signal to drive a small vehicle in the real world and controlling the attitude (both the position and rotation) of an object in 3D virtual space; 4) a flexible conductive textile sensor to detect the finger motion in terms of self-powered sensory information from textile as a novel human-machine interface towards virtual reality (VR) and Augmented Reality (AR) control applications.


    About the speaker

    Chengkuo Lee, PhD

    Dr. Chengkuo Lee received his Ph.D. degree from The University of Tokyo, Tokyo, Japan, in 1996. Currently, he is the director of Center for Intelligent Sensors and MEMS and an Associate Professor in the Department of Electrical and Computer Engineering at National University of Singapore, He cofounded Asia Pacific Microsystems, Inc. (APM), Hsinchu, Taiwan in 2001, where he was Vice President of R&D from 2001 to 2005. From 2006 to 2009, he was a Senior Member of the Technical Staff at the Institute of Microelectronics, A-STAR, Singapore. His research interests include MEMS, NEMS and flexible devices for energy harvesting, nanophotonics, metamaterials and biomedical applications. He has co-authored more than 280+ journal articles and 300+ conference papers. He holds 10 US patents and 26 Taiwan patents. His google scholar citation is more than 8300 and H-index is 47. He is in the Executive Editor Board of J Micromechanics and Microeng. (IOP, UK). He is the Associate editor of Journal of Micro/Nanolithography, MEMS and MOEMS (JM3; SPIE). He is also the Editor of Scientific Reports (Nature Publisher) and Editor of Journal of Sensors (Hindawi).

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    4:00 PM // Dr. Isabelle Bonne, Head Electron Microscopy Laboratory, Life Sciences Institute
    National University of Singapore
    Electron Microscopy, a fantastic tool for Biologists

    Electron Microscopy is a powerful imaging tool for analysis of composition, ultrastructure and function of biological samples and materials. From tissues and cells to subcellular structures and macromolecular complexes, Transmission and Scanning electron microscopy provides 2D and 3D morphological analysis. Studying and analysing structures in minute detail means we can learn more about how they function, leading to new insights into health and disease.

    The presentation will describe several key examples from case studies to illustrate the use of Electron Microscopy. In microorganisms, morphological characterization provided valuable insights into Chikungunya virus of epidemic potential, Enterovirus 71 infection of the brain in Hand, foot and Mouth disease, the role of pili in streptococci, the localization of UL44 protein in HCMV infection. Working with cells, we can observe how Drosophilia cells use tunneling nanotubes for transport, how Shigella survives in Hela cells, Listeria monocytogenes in zebrafish and Chikungunya virus in Mosquitoes.
    All are great examples of interactions between microorganisms and host cells and tissues.

    About the speaker

    Isabelle Bonne, PhD

    Dr Isabelle Bonne is a Cell Biologist in Electron Microscopy. She holds her PhD degree in Paris 7 University, France, working on the role of actin filaments in the mechanism of intracellular survival of Mycobacterium avium in macrophages. After a postdoctoral position in UCLA, USA, she joined the Electron Microscopy Facility at the Institut Pasteur in Paris, France, as a Research Engineer. During all these years, she focused on understanding physiological and pathological process through the use of advanced electron microscopy methods such as negative staining, cellular electron microscopy, cryo-electron microscopy and correlative microscopy. It is now over 4 years that Isabelle has been working as an imaging expert at the National University of Singapore. She joined the Life Science Institute in September 2016 to manage the Electron Microscopy Laboratory using different approaches in electron microscopy to study a large variety of biological samples ranging from macromolecules to tissues.

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    4:30 PM // Dr. Philipp Bastians, Product & Applications Sales Specialist
    ZEISS Research Microscopy Solutions
    3D SEM Technique for Bio-Medical Research

    Electron microscopy imaging has been used as a valuable research tool in the Life Sciences for many years. From research of single cell organisms, viruses or eukaryotic cells to identification of synaptic contacts between neurons, the ability to image biological samples in nanometer resolution has proven to be extremely valuable for many areas of biological research. The majority of these examples has been studied using the well-established Transmission Electron Microscopy (TEM) technique which requires thin sample preparations and limits the third dimension in scale and resolution.

    Recent developments in Scanning Electron Microscopy (SEM) opens the spectrum of high resolution and high-volume imaging of biological tissue. Based on sample block-face scanning in combination with milling (e.g. Focused Ion Beam (FIB)) or block-face cutting techniques, SEM enables dramatic resolution improvements in the third dimension above what is possible with TEM. Furthermore, SEM offers large field of view imaging and opens up applications that have previously been unachievable in the lab.

    In addition to the high volume and high-resolution imaging of SEM, FIB 3D SEM offers three-dimensional imaging of unstained cryo-frozen biological tissue opening the field for novel correlative studies using confocal microscopy. These include 3D colocalization of genetic markers and increase of context information for electron microscopy data.

    Further correlative applications utilize X-Ray Microscopy (XRM) to non-destructively assess sample structure prior to subsequent analysis using electron microscopy. The correlation of datasets across length scale and imaging modality (light microscopy to XRM to electron microscopy) extends the portfolio of applications for research that has previously been unachievable in the lab. In addition, pre-scanning assessment of biological samples prior to 3D electron microscope techniques such as serial sectioning SEM or Focused Ion Beam SEM reduces experiment failure rate and experiment duration.

    This talk will introduce some of the latest work in Life Science using 3D SEM techniques and discuss the future possibilities that imaging with this technology could provide.


    About the speaker

    Philipp Bastians, PhD


    • 2013-2018: University Munich, Germany, PhD
      Advisor: Prof. Dr. M. Helmstaedter, MPI Frankfurt
    • 2009-2012: University Heidelberg, Molecular Biotechnology,
      Degree: BSc


    • 2013 – 2018 Max-Planck-Institute for Brain Research, Frankfurt am Main, Germany, Ph.D.
      Thesis ”Comparative Cortical Connectomics: Circuit Analysis Across Species and Cortex Types” Department Connectomics, Dr. M. Helmstaedter
    • 2011 Keyence Germany GmbH, Neu-Isenburg, Germany, Internship in Sales and Distribution of Light Microscopes
    • 2011 – 2013 Max-Planck-Institute of Neurobiology, Munich, Germany, Research Assistant
    • 2010 - 2011 Max-Planck-Institute for Medical Research, Heidelberg, Germany, Student Assistant.
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  • Details


    27 February 2019, Wed
    8:45 AM - 5:15 PM

    National University of Singapore
    5A Engineering Drive 1, T-Lab Building
    Level 5 Seminar Rooms



    Mr. Fhu Chee Kong
    Mobile: +65 9170 7917

Day 2 | Workshop

Come and see the high resolution imaging of nanostructures, soft materials and life science samples using the smallest ion probe with contrast known from light ions.

Does your research require precision nanofabrication of devices at the nanoscale? Have you ever thought of using more than a Gallium Beam? Discover what it takes to fabricate precise, sub-10 nm structures that cannot be made using a gallium FIB alone.

Join us in this workshop to learn more about microscopy and nanofabrication with the ZEISS ORION NanoFab.

Maximum 5 persons per workshop. Registration is mandatory.


28 February 2019, Thur

Workshop Morning
9.00 AM - 12.00 NN

Workshop Afternoon
2.00 PM - 5.00 PM

National University of Singapore
4 Engineering Drive 3
NUS Engineering E4



Dr. Vignesh Viswanathan
Mobile: +65 9011 5965

Registration is closed

Due to overwhelming interest, we have closed the registration to the event. 

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