Life Science Series 2

July 26 2PM SGT

Join us for the virtual ZEISS Life Science Week (19 July - 2 August 2022), where we highlight the latest developments in microscopy and, more importantly, how these technologies have been utilised in day-to-day research.

Topics range from Laser Scanning Confocal Microscopy, Lattice Lightsheet imaging to Artificial Intelligence. For each topic, you can expect an informative presentation from ZEISS experts followed by engaging discussion from key-opinion leaders and an online demonstration.

Series Overview

 

Talks

Speaker(s)

Series 1

Deconvolution for Confocal Microscopy: ZEISS LSM Plus and Airyscan jDCV

Dr. Xianke Shi

Series 2

Imaging long term dynamics of subcellular structures in biological samples with the ZEISS Lattice Lightsheet 7

Mr. Gavin Symonds

Lattice Light Sheet Microscopy in A Core Facility Environment

Dr. Niall Geoghegan

ZEISS Lattice Lightsheet 7 in Imaging Facility 101

Dr. Sandra Fok

Series 3

The use of Artificial Intelligence in Light Microscopy

Dr. Mark Stafford & Dr. Shannon Das

MetaSystems and Machine Learning: Advances in Integrating Deep Numeral Networks into High Throughput Applications​

Mr. Benjamin Ching

Talk 1

Imaging long term dynamics of subcellular structures in biological samples with the ZEISS Lattice Lightsheet 7

Developments in Lattice Lightsheet technology have enabled researchers to gain new insights into the behaviour of their biological samples. Lattice Lightsheet brings the possibility of very gentle, long term, fast volumetric imaging as a tool to biomedical researchers, whilst at the same time teasing out subcellular structures that provide additional details of the biological structures at play. ZEISS has recently released the new Lattice Lightsheet 7 in a very compact but powerful, flexible and user-friendly instrument accessible to all biologists. The presentation will provide a general introduction to the range of samples that can be used with the Lattice Lightsheet 7, whilst also providing a deeper insight into the technology behind this new instrument. For example the Lattice Lightsheet 7 is unique because it is based on an inverted geometry and permits the use of all standard sample carriers including petri dish and multiwell slides/ chambers.

Speaker

Mr Gavin Symonds

Gavin graduated from The University of Sydney in 1995 with a degree in Science. ​​His interest in optical imaging developed from a project during his studies that ​​involved characterising the behaviour of a benchtop experimental multichannel ​​confocal microscope, and examining the resolution performance of immunogold ​​and silver labelled cells undergoing mitosis. Gavin joined ZEISS in 1995 and has ​​had a variety of roles including technical and application support. Gavin is currently ​​the product manager for the LSM/ Elyra/ Lightsheet portfolios.​​

Talk 2

Lattice Light Sheet Microscopy in A Core Facility Environment

In 2017 the Centre for Dynamic Imaging at WEHI launched their first, custom-built, Lattice light sheet platform. Since then, the platform has been applied to numerous projects such as malaria infection of red blood cells, cell death, cell migration, cell division and many other areas of research at the institute. The system gave unprecedented insights into the dynamic 3-Dimensional nature of cell biology answering challenging questions and leading to new discoveries. In 2020, the centre acquired a ZEISS LLS7 as part of their early access program. This commercial realisation of the lattice light sheet technology introduces a unique inverted optical geometry that allows researchers to image their live biological samples using traditional sample preparation methods and sample holders (i.e. 35mm petri dish). Fast but very gentle live cell volume imaging over extended periods of time is possible with this solution. The presentation will provide introduction to the lattice lightsheet concept and highlight unique ZEISS implementation with application examples. In this talk Niall will give an overview of how the lattice technology sits within a core facility environment, enabling new discoveries across a diverse range of samples and diseases.

Speaker

Dr Niall Geoghegan

Dr Niall Geoghegan is a Senior Postdoctoral Researcher in the Rogers Lab and Imaging Specialist within the Centre for Dynamic Imaging (CDI) at WEHI. He completed a Masters in Electronic and Electrical Engineering at the University of Glasgow in 2010, followed by a PhD in Biomedical Engineering in 2015. During his PhD he focussed on developing time-resolved microscopy techniques to explore the physical properties of cell membranes. In 2016 he joined the CDI at WEHI to construct and run the institute’s first lattice light sheet microscope. Since then, he has helped apply this technology to numerous areas of the institute’s research from cell death to infectious disease.

Talk 3

ZEISS Lattice Lightsheet 7 in Imaging Facility 101

Situated within The University of New Wales (UNSW, Kensington campus) is the Katharina Gaus Light Microscopy Facility (KGLMF). The facility which caters to UNSW and external researchers and students has over 40 imaging instruments including but not limited to fully enclosed epifluorescence microscopes for cell live imaging (ZEISS CD7), confocal microscopes with Airyscan providing greater resolving power to super-resolution microscopes (ZEISS Elyra 7 with Lattice SIM) for that fast but gentle live cell experiment. In this talk, we discuss how the ZEISS Lattice Lightsheet 7 microscope integrates within our large facility, the model we use to hand pick projects fitted for Lattice Lightsheet imaging. Then followed by showcasing a range of samples and applications using this system, together with the challenges met and resolutions. This talk will build a foundation for researchers to enter the Lattice Lightsheet imaging world.

Speaker

Dr Sandra Fok

Sandra Fok is Biomedical Imaging Specialist in the Katharina Gaus Light Microscopy Facility within the Mark Wainwright Analytical Centre. Her key role is in advanced light and optical imaging, where she leads several collaborative microscopy related research projects in specimen preparation, acquisition, and analysis. In addition, she provides research support and trainings. She is known for her experience in lightsheet microscopy (Gaussian and Lattice) and oversees the operation of the lightsheet labs in the facility. Her research interest is in cancer migration. More specifically understanding the physical, cellular, and molecular determinants in cancer cell invasion in high dense matrices. Lightsheet, intravital imaging (mouse and zebrafish), live cell imaging, superresolution, slide scanning and spectral imaging are some her of favourite imaging techniques.

Register for our Life Science Week to get a chance to sign up for our limited online demonstration sessions