ZEISS Correlative Cryo Workflow

Your Solution for TEM Lamella Preparation and Volume Imaging under Cryogenic Conditions

Cryogenic microscopy allows the examination of cellular structures in their near-to-native state, as the ultrastructure of cells and tissues can be preserved free of artifacts and cellular processes are stopped instantaneously. However, cryogenic microscopy presents users with complex challenges, such as time-consuming preparation and imaging procedures, devitrification, ice contamination or loss of samples – even more if you want to correlate the obtained data across imaging modalities.

ZEISS Correlative Cryo Workflow connects widefield, laser scanning, and focused ion beam scanning electron microscopy in a seamless and easy-to-use procedure. The solution provides hardware and software optimized for the needs of correlative cryogenic workflows, from localization of fluorescent macromolecules to high-contrast volume imaging and on-grid lamella thinning for cryo electron tomography.

Correlative Cryo Workflow accessories

Imaging the near-to-native state

  • Seamless cryogenic workflow across multiple modalities
  • Sample protection against devitrification and ice contamination
  • High-resolution fluorescence imaging
  • High-contrast volume imaging and 3D reconstruction
  • Targeted on-grid lamella thinning for cryo TEM applications
  • Multipurpose use for cryogenic and room temperature applications

A simplified workflow to help you focus on your research

With ZEISS Correlative Cryo Workflow, you master the challenging combination of different imaging modalities under cryo conditions. The workflow solution connects light and electron microscopy, enabling volume imaging and efficient production of TEM lamellae. Dedicated accessories simplify the workflow and facilitate a safe transfer of cryo samples between the microscopes. Data management is assured by ZEN Connect, which keeps your data in context throughout the workflow. A series of processing tools help you enhance the imaging results.

Correlative Cryo Workflow in the Lab
Double-labelled yeast cells (CNM67-tdTomato and NUP-GFP).
Double-labelled yeast cells (CNM67-tdTomato and NUP-GFP). LSM image (left) and Crossbeam image (right). Sample courtesy M. Pilhofer, ETH Zürich, Switzerland

Superior components to give you best-in-class data quality

Thanks to cryo-compatible objectives and the high sensitivity of the Airyscan detector, ZEISS LSM systems enable you to detect proteins and cellular structures at high resolution while gentle illumination and constant low temperatures prevent your samples from devitrification. The ZEISS Crossbeam FIB-SEM lets you enjoy high-contrast volumetric imaging – even without heavy metal staining applied to your samples. Both modalities provide valuable functional and structural information that can give you a thorough understanding of ultrastructure, whether or not you follow up with TEM studies.

Multipurpose solutions to maintain your imaging facility’s productivity

Unlike other solutions, the ZEISS microscopes involved in the workflow can be used not only for cryogenic microscopy, but also for room temperature applications, which is particularly advantageous when the microscopes are not being fully utilized for cryogenic experiments. Converting the instruments from cryogenic to room temperature usage is done quickly and doesn’t require technical expertise. This flexibility gives users more time for their experiments. Imaging facilities benefit from better utilization and a faster return on investment.

Core Imaging Facility with Cryo equipment

ZEISS Correlative Cryo Workflow at a glance

Correlative cryo-workflow

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