ZEISS On Your Campus (ZOYC) Online is comprised of free live online webinars with your local account team.
ZOYC Online has three main goals:
1. Provide education focused on better utilisation of your current microscopy equipment, which can lead to:
- Higher quality imaging and faster time to results
- A better understanding of the data that are collected
- Improved experimental design
2. Bring awareness of new and emerging microscopy trends and technologies.
3. Connect live with your local ZEISS account team.
Samuel Ko, BSc (Hons.), MPhil., PhD
Head of Product and Application Sales Specialist
Dr Samuel Ko studied Biochemistry in Hong Kong with a particular focus on the cellular response of tumour necrosis factor-alpha-induced apoptosis in mouse fibroblast cell line L929 during his MPhil and PhD research, by using fluorescent imaging techniques such as Confocal Laser Scanning Microscopy (CLSM). He received his PhD from The Chinese University of Hong Kong in 2001. From 2001 to 2005, he worked as a post-doctoral fellow at Department of Surgery, University of Hong Kong, where he investigated carcinogenesis of gastric adenocarcinoma. Since August 2005, Samuel joins Carl Zeiss Singapore as a regional application specialist. He is mainly in charge of the products of CLSM, SuperResolution Microscopy, Lightsheet Fluorescence Microscopy (LSFM) and PALM MicroBeam LCM (Laser Capture Microdissection).
Very often in life sciences research, to reach a reliable result, it is necessary to perform multiple repetitions of experiments or to run complex assays. Automation and parallelisation are of significant assistance in reducing the time it takes to achieve this. This drive towards automation and efficiency resulted in the development of Celldiscoverer 7 to combine the easy-to-use automation of a boxed microscope with the image quality and flexibility of a classic inverted research microscope. Celldiscoverer 7 calibrates itself, automatically detects and focuses your sample and optimises all optical parameters without the requirement of any user interaction. All Celldiscoverer 7 components are optimised for hassle-free automated imaging. New users and multi-user facilities will particularly enjoy the in-built automation, and usability features since even the most inexperienced of microscope users can capture high-quality data with ease. This leaves more time to get on with other projects.
We now proudly present the Celldiscoverer 7 with LSM 900 and Airyscan 2, which fuses the advantages of widefield and confocal imaging. The unparalleled sensitivity of the camera, as well as the LSM detectors, guarantee for the gentlest image acquisition when working with sensitive biological samples. Airyscan 2, with its new Multiplex mode, enables the acquisition of large volumes with high temporal and optical resolution. Also, we present Mixed Mode, where camera acquisition and confocal imaging can now be combined in the same experiment.
The principle goal of ZEISS Celldiscoverer 7 with LSM 900 is to make complex microscopy simple. Whether working with 2D or 3D cell cultures, tissue sections or small model organisms, you will benefit from better data in a shorter time with this reliable automated research platform.
Among the most critical artefacts to consider in widefield fluorescence microscopy arises from, the fact that regardless of the focal point, illumination from the objective produces fluorescence throughout the entire specimen volume.
Imaging of thick specimens in fluorescence microscopy is then compromised by signal originating from regions above and below the focal plane. The result is that sharp image information from the focal plane is overlaid with blurred image information arising from a distant area, reducing contrast and resolution in the axial (z) dimension. Furthermore, three-dimensional (3D) reconstruction of the specimen is not possible under these conditions.
Aside from using laser confocal technique, this webinar explores how to get better fluorescence images with your widefield microscope by different image post-processing methods as well as using the structured-illumination technique with Apotome.2
Modern X-ray microscopy (XRM) combines the many remarkable characteristics of X-rays; the penetrating power and the quality to diffract off of the crystalline lattice planes (traditionally used in XRD) to interrogate interior structures of samples nondestructively providing scientists with X-ray vision via radiography or tomography within a single laboratory-based instrument. Following a trend somewhat analogous to that which was encountered previously in Scanning Electron Microscopy (SEM), wherein different electron contrast methods complemented with EDS and EBSD techniques turned the SEM from an imaging-only tool into a robust analytical platform, XRM is now expanding well beyond the classical limitations of X-ray CT or microCT. Specifically, XRM is extending the range of imaging modalities (now including phase contrast in addition to the well-known absorption contrast) and incorporating analytical diffraction information from polycrystalline samples.
Based on a technique initially developed at a few select synchrotron facilities worldwide, diffraction contrast tomography (DCT) is now available in the lab. It leverages both the absorption-based and diffraction information from X-rays’ interactions with a sample to reconstruct the crystalline microstructure in 3D. As a nondestructive method, this new analytical technique offers us the opportunity to observe phenomena which were never before possible in 3D: such as grain growth through annealing, studying the local effects of corrosion, coupling mechanical behaviour with local grain structure, or correlating to complementary methods like 2D/3D EBSD. This webinar will introduce LabDCT using examples, and how it complements and extends the range of imaging opportunities provided by XRM, spanning from materials to life sciences.
Fluorescence microscopy allows researchers to study the structure and function of the brain in both fixed samples and in vivo. Laser scanning microscopy, confocal and multi-photon, serve as the standard imaging approaches for imaging into scattering samples. However, due to the light scattering properties of brain tissue, LSM suffers in both depth penetration and resolution. By combining the unique Airyscan detection concept with multi-photon excitation, the resolution, signal-to-noise, and speed benefits of Airyscan can be extended to deeper layers of the cortex (2-3x deeper than traditional confocal). Also, combining Airyscan with GRIN lens technology enables increased resolution and signal-to-noise while imaging regions of the brain that are unreachable with conventional in vivo microscopy.
To add additional context to the data, Airyscan imaging through a GRIN lens can be correlated with freely behaving imaging. Miniature microscopes such as the Inscopix nVista™ and nVoke™ systems can be used for cellular-resolution imaging and optogenetic manipulation in freely behaving animals to study functional network activity. The methodology has been developed to register the same neurons imaged with the Inscopix miniature microscopes to neurons imaged with Airyscan. Hardware and analysis tools have been developed that enable recording from the same focal plane between the two modalities and correct for the different scale, rotation, and elastic deformations between the images. The ability to register data from freely behaving imaging experiments to high-resolution Airyscan images provides crucial links between activity dynamics and anatomical, molecular, and/or connectivity profiles of distinct neuronal populations and can enable exciting new insights into brain health and disease.
The LaserFIB combines an ultra-short pulsed laser, typically a femtosecond (fs) laser, and a FIB-SEM, all in one microscope. Massive material ablation by the laser allows to gain rapid access to structures buried deeply in, e.g. packaged electronics or display devices. FIB-SEM can then analyze the targeted regions of interest. Remarkably, sample damage or heat effects induced by the laser are minimal. Thus, the LaserFIB is attracting attention also in the field of materials engineering and characterization, e.g. for the fabrication of micromechanical testing devices with dimensions of up to millimetres or large cross-sections for EBSD.
As a leading supplier of electron and ion-optical systems, ZEISS offers state of the art Secondary Ion Mass Spectroscopy (SIMS) technology for compositional and Isotopic analysis. The webinar will give an overview of the high-end SIMS technology and its potential application space.
In recent years, X-ray microscopy (XRM) has grown out of origins at synchrotron facilities and has set new benchmarks in high resolution, nondestructive 3D characterization. With the 3rd and now 4th generation synchrotrons, and accompanied by exponential improvements in both processing power and beam quality, recent progress at these tomography beamlines has been made possible. Furthermore, an expansion in the variety of imaging/spectroscopy modalities has created increasingly rich and descriptive data sets with newly integrated correlative imaging workflows.
Many of these techniques have also translated to a broader community via analogous lab-based machines. Through incorporating synchrotron-style optics, lab-based XRM systems can now achieve comparable levels of resolution and contrast, moving CT beyond an inspection/NDT technique and well into the scientific realm. Also, similarly to the synchrotron, in situ imaging in the lab has become more prevalent as well, albeit at a different time scale. It has been demonstrated now that, for some specific experiments, lab systems can be more suitable. Lastly, the classical absorption tomography of CT or microCT is being supplemented with an increasing range of modalities available on lab XRM systems, most notably and recently that of diffraction contrast tomography (DCT).
This presentation will explore these emerging laboratory-based methods, namely in situ and diffraction contrast tomography, and provide examples of their application in materials science along with an expansion of the classic “single-instrument” microscopy to correlative approaches linking XRM and 3D SEM.
Recent years have witnessed major progress in three-dimensional (3D) microscopy techniques for the life sciences as well as materials research. In particular, scanning electron microscopy (SEM) offers new insights into the 3D organization of cells and tissues by volume imaging methods, such as array tomography, serial block-face imaging or focused ion beam FIB-SEM tomography. Recently, a novel multi-beam SEM technology for imaging of large sample areas has been developed by ZEISS. The MultiSEM family features 61 or even 91 electron beams scanning in parallel, resulting in an imaging speed of up to 1820 megapixels per second. At this rate, the MultiSEM family currently provides the fastest scanning electron microscopes in the world. Complemented by the advent of automated sample preparation robots, the promise of mapping larger (1 mm³) tissue volumes at high-resolution is now within reach.
This finally strengthens a new field within the Neurosciences, namely Connectomics, where complex synaptic networks assembled by billions of neurons are studied. Structural analysis is done by first slicing neural tissue followed by imaging the sections using a scanning electron microscope. After merging all section images into a 3D volume, the fine structure of neurons can be segmented and visualized. The result is a detailed 3D map of the connectivity of the brain: the Connectome.
This talk will outline some of the challenges in Connectomics and explain recent technological developments. The focus will be given to ZEISS solutions for array tomography and high-throughput electron microscopes such as the ZEISS MultiSEM.